The amniotic membrane should be thawed before use for 30 minutes at room temperature. The color of the storage medium after thawing can vary from light pink to orange tones.
AmnioVet® is presented in a double bag. Using delicate forceps or sterile gloves, the inner bag containing the amniotic tissue is removed. This inner package can be put in contact with the sterile field. With scissors, the package is cut below the sealing line of the inner bag and the piece of tissue is carefully removed with sterile forceps, avoiding membrane to come in contact with the inside of the bag and is attached to it. Once extracted, AmnioVet® can be easily removed from the nitrocellulose support with a surgical sponge dry or cornea forceps. One should generously wash the AmnioVet® with saline solution in order to remove any residual cryopreservative fluid.
The tissue is immediately ready for transplantation after separating it from the supporting nitrocellulose. There is no need for rehydration of the tissue. AmnioVet® is always presented with the side of the amniotic stroma adhered to nitrocellulose paper. After removal of this support, one can easily determine the orientation of AmnioVet® using a surgical sponge dry, which will adhere to the side of the stroma, but will not stick to the part of the basement membrane.
The processing and cryopreservation method that we use in Vetbiologicals, allows us to produce an amniotic membrane that retains the tissue’s natural characteristic tensile strength, which makes AmnioVet® relatively strong, easy to handle and to suture.