What is AMNIOVET™?

AMNIOVET™ is a cryopreserved biomaterial from bovine amniotic membrane. It has been processed under strict aseptic conditions, packaged in a double polyethylene envelope with cryopreservative liquid and subjected to a controlled freezing down to -78º C.

Histology of AMNIOVET™

The thickness of AMNIOVET™ ranges from 50 to 90 μm. The histology of AMNIOVET™ is very similar to that of the cornea. To repair the full thickness of a cornea (400-500 μm), 4 to 10 single layers of AMNIOVET™ will be required.

AMNIOVET histology, fresh amniotic membrane

Histology of fresh amniotic membrane AMNIOVET™.

Histological image of the dog cornea

Histological image of the dog cornea: epithelium (1), stroma (2), Descemet membrane (3) and corneal endothelium (4).

Source: Coyo, N., Leiva, M., Peña, T. The corneal endothelium and its main diseases in the dog. Clinics. Vet. Peq. Anim., 2017, 37 (3): 171-180

Why use AMNIOVET™?

AMNIOVET™ offers the veterinarian an exceptional solution for tissue repair and regeneration in ocular surface surgery.

  • Preserves maximum transparency
  • Ease of handling: resistant, malleable, adaptable to the cornea
  • Thickness suitable for single and multilayer application
  • Rich in growth factors and cytokines
  • Unique biological activities:
    • Promotes corneal re-epithelialization
    • Reduces inflammation and pain
    • It has an antimicrobial effect.
    • Inhibits collagenase activity
    • Decreases vascularization
    • Minimizes scarring
    • Very low antigenicity
Indications for use of AMNIOVET™

AMNIOVET™ can be used as a corneal coating. (cover, overlay) or as a graft (graft, inlay). As a coating it protects the ocular surface and facilitates corneal re-epithelialization. If applied as a monolayer or multilayer graft, AMNIOVET™ acts as a matrix for epithelial cell growth. Both techniques can be combined in the same surgery.

  • Anterior stromal ulcers
  • Corneal dermoids
  • Corneal abduction
  • Pigmentary keratitis
  • Corneal/intraepithelial neoplasms
  • Corneal dystrophies/degeneration
  • Deep corneal ulcers
  • Corneal perforations
  • Collagenase ulcers (melting)
  • Indolent ulcers (SCCED)
AMNIOVET™ Presentations

AMNIOVET™ is available in 40 × 40 mm and 25 × 25 mm sizes.
Each membrane is attached to a nitrocellulose paper, fixed on a support, and individually packed in a double sterile polyethylene bag with cryopreservative liquid.

How is AMNIOVET™ distributed?

AMNIOVET™ is shipped throughout Europe in isothermal boxes with dry ice, with express courier service in 24-48 h.


Dry ice or carbonic snow is carbon dioxide (CO2) in solid form. When sublimed, it leaves no moisture residue because its base is not water and its natural state is gaseous. Dry ice is extremely cold and can cause severe frostbite. It is important to handle dry ice with gloves and safety glasses.

Dry ice should be left in a ventilated place as it converts to CO2 on sublimation and there is a risk of asphyxiation.





  • Do not ingest.
  • Cold and pressure can be dangerous.
  • Keep out of reach of children.
  • Keep in a ventilated place, especially in low areas.
  • CO2 has a higher density than air and falls to declining areas.
  • Do not handle in basements or enclosed or small rooms.
  • Keep container below 50° C.
  • Do not seal a container containing dry ice.
  • Once the contents have been removed, replace the lid of the UNSEALED container and keep in a well-ventilated place until complete evaporation.
  • CO2 gas is non-flammable, odorless and tasteless. Non-toxic.

How should AMNIOVET™ be stored in the clinic? Expiration

AMNIOVET™ can be stored for up to 1 year in a standard freezer at -18°C.

Preparation of AMNIOVET® for surgery:
1. Thaw AMNIOVET® at room temperature for approx. 15 minutes.

2. In a sterile environment, open the outer envelope.

3. The surgeon will remove the sterile (!) inner envelope and open it with scissors. With forceps he will remove the support with the membrane and leave it in a container where he will gently irrigate it with saline solution to eliminate the cryopreservative liquid.

4. The cryopreservative liquid from the sachet is collected in a sterile bottle (e.g. urine bottle). In case of not using the whole membrane, the remaining piece can be kept in this bottle in the refrigerator for a maximum time of 24h. DO NOT REFREEZE.

Epithelial side and stromal side

AMNIOVET™ is adhered to the nitrocellulose paper on its stromal side.

The epithelial face may be covered by a more or less profuse mucus, which adheres to a haemostatic. If there is a lot of mucus, it can be removed with a blunt instrument or a microsponge. In some cases it may be advantageous to maintain the mucus to take advantage of its growth factors and cytokines. In these cases AMNIOVET™ will be used. The epithelial side with the mucus will be placed over the cornea like a protective lens and the epithelial side with the mucus will be placed over the cornea.

If we want the amniotic membrane to be integrated into the cornea, AMNIOVET™ will be placed with the stromal side touching the surgical bed and the epithelial side facing up.

If we want to use the membrane as a protective lens, we will place the epithelial side over the cornea.

How to trim AMNIOVET™?

To facilitate cutting AMNIOVET™ to the desired size, it is advisable to do so while it is still adhered to the nitrocellulose paper.

AMNIOVET™ Tensile Test


Opening of the AMNIOVET™ envelope.


Preparing AMNIOVET™ for surgery.