Vetbiologicals ico


AMNIOVET™ offers a unique tissue repair and regeneration solution for the management of many ocular surface diseases.

AMNIOVET™ is a biomaterial based on bovine amniotic membrane that has been obtained and processed through a refined and meticulous cryopreservation process. Unlike other methods of preservation, cryopreservation retains the valuable activity of cytokines and growth factors amnion. This confers unique biological activities that stimulate tissue repair, minimize scar formation, reduce inflammation and control ocular pain. Amniotic membrane grafts have been shown to be of particular value in ophthalmic surgery.

AMNIOVET™ is an easy-to-use therapeutic option that can be comfortably fixed to the surgical site with sutures or tissue adhesives.

Vetbiologicals ico


The amniotic membrane is obtained under sterile conditions by elective cesarean section from cows with uncomplicated pregnancies. Donor females are examined to confirm a general condition healthy and free of infectious diseases.

The placenta is immersed in a sterile solution with antibiotics and antifungals, and is transported to the laboratory immediately. Here, the amniotic membrane is mechanically separated from the placenta. Under a laminar flow hood, the membrane is washed with a sterile solution of antibiotics and then stored adhered to a nitrocellulose support with the surface of the epithelial layer / basement membrane side up.

During processing of the amniotic membrane, samples are collected in order to perform random microbiological control cultures.

AMNIOVET™ is packaged in individual amniotic membrane/nitrocellulose units (at a size of 4×4 cm) in a double sterile polyethylene bag and immersed in a cryopreservative liquid. The packaging is sealed and subjected to a controlled freezing rate down to -78ºC.

AMNIOVET™ is shipped in thermal boxes with dry ice. After delivery, samples can be stored for up to 1 year in a standard household freezer (-18°C). When the tissue is thawed, the amniotic membrane should be used immediately.

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The amniotic membrane should be thawed before use for 30 minutes at room temperature. The color of the storage medium after thawing can vary from light pink to orange tones.

  • AMNIOVET™ is presented in a practical format as a double bag.
  • Using delicate forceps or sterile gloves, the inner bag containing the amniotic tissue is removed. This inner package can be put in contact with the sterile field.
  • With scissors, the package is cut below the sealing line of the inner bag and the piece of tissue is carefully removed with sterile forceps, avoiding membrane to come in contact with the inside of the bag and is attached to it.
  • Once AMNIOVET™ has been removed, the amniotic membrane can be easily separated from its supporting paper with a dry surgical sponge (hemostatic) or corneal forceps. One should generously wash the AmnioVet® with saline solution in order to remove any residual cryopreservative fluid.

The tissue is ready for transplantation immediately after separation from the backing paper without the need for rehydration. AMNIOVET™ is always presented with the amniotic stroma side attached to the nitrocellulose paper. Once removed from this support, it is also easy to determine the orientation of the AMNIOVET™ using a hemostatic: the dry surgical sponge will stick to the stromal side, but will not stick to the basement membrane side.

The processing and cryopreservation method we use at Vetbiologicals allows us to produce an amniotic membrane that retains its characteristic natural tensile strength, making AMNIOVET™ a relatively strong tissue that is easy to handle and suture.